David Weir and Sarah Storey, who each won four gold medals at the London 2012 Paralympics, will carry the British flag together in the closing ceremony on Sunday night.
The move is a change to the normal protocol of selecting a single individual, and team officials had to gain permission from the International Paralympic Committee to make the move.
"During the London 2012 Paralympic Games, ParalympicsGB has witnessed some outstanding performances, and most notably from Sarah Storey and David Weir," said chef de mission Craig Hunter. "I'm thrilled and delighted that the IPC has recognised and agreed to our request to allow joint flagbearers at the closing ceremony."
He added: "These athletes represent the spirit of Paralympic athleticism."
Wheelchair racer Weir has lit up the Olympic Stadium over the last 10 days, with victories over 5000m, 1500m and 800m and won Britain's last gold of the Games in the T54 Marathon on Sunday morning.
At the end of his fourth Games, the 33-year-old Londoner now has a career haul of six gold, two silver and two bronze medals.
"It will be a great honour to come out to the closing ceremony carrying that flag," said Weir. "I'm a proud Brit and Sarah is as well. She has done fantastically well too. "To do it as a Londoner in front of a London crowd makes it even more special."
Storey starred in the Velodrome and on the road, taking individual pursuit and 500m time trial victories on the track before moving to Brands Hatch where she won the Time Trial and triumphed in emphatic fashion in the C5 Road Race on Thursday.
That win brought her 11th career gold, making Mancunian Storey - a former swimmer who switched to cycling in 2005 - Britain's most decorated female Paralympian. With eight silver medals and three bronze in a 20-year career she surpassed former wheelchair racer Baroness Tanni Grey-Thompson's collection of 11 golds, four silvers and one bronze.
"I'm amazed at the news, I never expected it," said Storey, 34. "I've not been to a Paralympic ceremony since Athens and can't wait to see the stadium tonight. It's a huge honour and it should be a very exciting night."