A nightly 15-minute light show will be beamed from the top of the giant twisting tower that stands near the Olympic Stadium, the London Legacy Development Corporation said.
A series of 250 coloured spotlights is being attached to the 114.5m tall ArcelorMittal Orbit, billed as the UK's largest sculpture,
These will be individually controlled in an array of digital combinations from the Anish Kapoor and Cecil Balmond designed structure. Passers-by will see both static and animated effects.
LLDC chief executive Andrew Altman said: "The feature lighting opens a completely new artistic aspect to the work of Anish Kapoor and Cecil Balmond.
"It will create a vivid landmark with dynamic effects that we can use in tandem with different events."
The external spiral staircase, the looping lattices, the canopy and viewing platforms areas are some of the areas where the energy efficient LEDs will be set.
There will be a 15-minute moving light show every evening, with special effects also created for major events in and around the Olympic Park in Stratford, east London.
The lighting will be tested over the next two weeks.
There will be views across London from the Orbit visitor attraction which stands between the Olympic Stadium and the Aquatics Centre.
Up to 5,000 visitors a day are expected, with a potential to attract around one million people during the first year of operation, when it reopens after the Games in 2014.