Kelly Sotherton's hopes of qualifying for the London Olympics suffered a major blow when she failed to finish the fourth event of a heptathlon in Italy.
Sotherton pulled up during the final discipline of the opening day, the 200m, as she looked to achieve the 'A' qualifying standard of 6,150 points.
It remains to be seen whether the Athens bronze medallist has suffered a serious injury, but time is already running out for the 35-year-old, who initially retired from the heptathlon and switched to the 400m before returning to the heptathlon last year.
Sotherton was down in fifth place after three events with 2,589 points, clocking 13.96 seconds in the 100 metre hurdles, clearing 1.72m in the high jump and throwing 12.98m in the shot.
That left her 71 points behind compatriot Katarina Johnson-Thompson, with the 19-year-old from Liverpool then moving into the overall lead in the competition with a personal best of 23.78secs in the 200m.
Johnson-Thompson ran a personal best of 13.68s in the hurdles, cleared 1.81m in the high jump and threw another personal best of 11.75m in the shot.
Her total of 3,662 left her 66 points ahead of Brazil's Lucimara da Silva, with another Briton Louise Wood in fifth on 3,443.