Great Britain's Dwain Chambers suffered a setback after missing both the A and B Olympic qualification standards in the 100 metres in Ostrava..
Eligible to compete at London 2012 after a change to the rulings relating to doping offenders, the 34-year-old was a distant fifth in 10.28 as Usain Bolt eased to victory. The fastest man of all-time got off to a poor start but sharpened up over the closing 30m, recording a time of 10.04 seconds.
In the other 100m race, GB's Mark Lewis-Francis crossed first in 10:36.
Three British hurdlers all ducked under the ceiling of the A standard in the 110m hurdles, with Andy Pozzi fourth (13.36), Lawrence Clarke fifth (13.42) and Andy Turner a disappointing sixth (13.48) in a race won by Dexter Faulk. The trio have met the A standard before.
Richard Davenport was fourth fastest in the 400m hurdles - his 51.54 almost two seconds slower than the winner, Brent LaRue, but a personal best five metres 72 saw Steven Lewis finish third in the pole vault.
Oscar Pistorius swapped the Paralmypic World Cup in Manchester for the 400m track here, but was a comfortable last in 47.66 as LeShawn Merrit demonstrated his class with a race-winning 45.13. Andrew Osagie impressed in the 800m, finishing second to Adam Kszczot in 1.45.24, while Andrew Baddeley was third in the 3000m.
In the women's events, five-time Olympic medalist Veronica Campbell-Brown was almost half a second quicker than her nearest challenger in the 200m, finishing in 22.38, while defending Olympic champion Christine Ohuruogu met the A standard in the 400m.
She recorded a time of 51.59 in one of her best runs over the last year, but she still came second to Sanya Richards-Ross. GB's Shana Cox was eighth and last.
Caster Semenya was second in the 800m, the 2008 gold winner edged out by Pamela Jelimo - GB's Marilyn Okoro was fifth while Dawn Hunt did not finish.
National coach Charles Van Commenee will have enjoyed seeing Tiffany Porter - his captain at the World Indoors in Turkey - win the 110m hurdles in 12.65, while Perri-Shakes Drayton met the B standard in finishing third in the 400m hurdles.